HPLC Column, NP Amino, 3um, 2.1mm ID x 50mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino™ is a normal-phase and HILIC stationary phase designed for the retention and separation of highly polar compounds. Manufactured using a three-carbon propyl ligand with a terminal amino functional group bonded to high-purity silica, this stationary phase provides selectivity that is particularly useful for carbohydrates, organic acids, and other polar analytes. As a USP L8 equivalent phase, it is widely used as an alternative in established USP methods and for new method development involving Normal Phase and HILIC chromatography.
68340-05P-2 is an HPLC column packed with 3 µm NP Amino™ particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. This short, narrow-bore configuration is optimized for rapid analyses, reduced solvent consumption, and compatibility with LC-MS workflows. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, the HPLC system must be capable of precise and reproducible low-flow operation.
This column is particularly useful for the analysis of sugars, carbohydrates, compounds containing carboxylic acid groups, and other polar molecules. Suitable for both Normal Phase and HILIC chromatography, the amino functionality provides retention characteristics that make it valuable for analytes that are poorly retained on conventional reversed-phase columns. As an unmodified silica bonded amino phase, it is also suitable for HILIC applications.
Key Benefits
NP Amino™ stationary phase
USP L8 equivalent chemistry
Amino bonded phase
High-purity silica support
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced retention of polar compounds
3 µm particle size
Greater efficiency than 5 µm columns
Reduced solvent consumption
Typical Applications
USP L8 methods
Sugar analysis
Carbohydrate analysis
Organic acid analysis
Polar compound analysis
HILIC methods
Normal Phase chromatography
Food and beverage analysis
Pharmaceutical analysis
Method development
Specifications
Product Type: HPLC Column
Stationary Phase: NP Amino™
USP Classification: L8
Chromatography Modes: Normal Phase and HILIC
Bonded Phase: Amino
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 100 Å
Column Dimensions: 2.1 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for highly polar compounds
Commonly used for sugars and compounds containing carboxylic acid groups
Suitable for both Normal Phase and HILIC chromatography
As an unmodified silica bonded amino phase, this column is suitable for HILIC applications
The 2.1 mm ID format provides reduced solvent consumption and is compatible with LC-MS systems
Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
The 50 mm length is ideal for rapid analyses and high-throughput methods
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Packed and shipped in Normal Phase solvents